Kinetic study on lipase-catalyzed esterification in organic solvents
نویسندگان
چکیده
A twin inhibition is observed for the esterification reaction between ethanol and isovaleric acid using immobilized lipase from Rhizomucor miehei in hexane and in mixed solvent system. The observed bi-substrate inhibition pattern follows a Ping-Pong Bi-Bi mechanism with dead-end inhibition of enzyme by both the substrates. An increase in Km value for alcohol in mixed solvent (0.645 M) than in hexane (0.256 M), indicates that the enhanced solvation of ethanol in mixed solvent results in lower degree of inhibition.
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